Thermo Fisher Scientific PINK1 Monoclonal Antibody (N4/15), FITC
PINK1 단백질을 검출하기 위한 FITC 결합 단클론 항체로, Western blot, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. 단백질 G 정제, 1 mg/mL 농도, 보존제 무첨가. 4°C 암소 보관, 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (IHC) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N4/15 |
| Immunogen | Fusion protein amino acids 112–496 (cytoplasmic C-terminus) of human PINK1 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.09 mM sodium bicarbonate/PBS (pH 7.4), 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932110 |
Additional Formats
- APC (Cat. No. MA5-45655)
- PerCP (Cat. No. MA5-45657)
- Custom conjugation available upon request
Product Specific Information
- 82% identical to rat and 81% identical to mouse; >30% identity with DMPK
- 1 µg/mL of MA5-45656 detects PINK1 (~50 kDa) in 20 µg rat brain lysate by colorimetric immunoblot using Goat anti-mouse IgG:HRP
- Formerly sold as clone S4-15
Target Information
PINK1 (PTEN-induced putative kinase 1) protects cells from mitochondrial dysfunction by regulating autophosphorylation activity of mitochondrial genes. The kinase domain is critical for its protective effects. Mitochondrial proteins are essential for cellular energy regulation and stress adaptation, especially in brain cells where energy demand is high. Dysfunction in mitochondrial structure or function can lead to neurodegenerative diseases such as Parkinson’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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