Thermo Fisher Scientific PINK1 Monoclonal Antibody (N4/15), APC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (IHC)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

N4/15

Immunogen

Fusion protein amino acids 112-496 (cytoplasmic C-terminus) of human PINK1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PINK1,uniProtId:Q9BXM7-1,ncbiNodeId:9606,antigenRange:112-496,antigenLength:581,antigenImageFileName:MA5-45655_PINK1_Q9BXM7-1_House_mouse.svg,antigenImageFileNamePDP:MA5-45655_PINK1_Q9BXM7-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

APC APC APC

View additional formats

• FITC
• PerCP
• Request custom conjugation

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

2.48mM MES/95.64mM phosphate, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932109

Product Specific Information

82% identical to rat and 81% identical to mouse. >30% identity with DMPK.

1 µg/mL of MA5-45655 was sufficient for detection of PINK1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 50kDa.

This antibody was formerly sold as clone S4-15.

Target Information

PINK1 (PTEN-induced putative kinase 1) protects from mitochondrial dysfunction in cells by conferring different autophosphorylation activity to mitochondrial genes. The kinase domain of PINK1 appears to be critical in its protective effects. Mitochondrial proteins are critical for the regulation of cellular energy and adaptation to stress. Particularly in brain cells, mitochondrial demand is extraordinarily high, in order to accommodate aerobic and anaerobic support of high energy processes. When mitochondrial structure and/or function is disrupted in the brain, neurodegenerative diseases like Parkinson`s disease are triggered.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.