Thermo Fisher Scientific CNOT4 Monoclonal Antibody (OTI1H2), TrueMAB
CNOT4 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI1H2). Western blot과 IHC(P)에서 검증됨. E3 ubiquitin ligase 활성을 가진 CNOT4 연구용에 적합. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1H2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 190–455 of human CNOT4 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
CNOT4 is a 575 amino acid protein with E3 ubiquitin ligase activity, involved in protein modification and ubiquitination.
It contains a C4C4-type RING zinc finger domain, an RNA recognition motif, and a bipartite nuclear localization signal.
CNOT4 is a component of the CCR4-NOT (CAF1) core complex that regulates transcription of multiple genes.
It interacts with CNOT1 via its C-terminus and with E2 ubiquitin ligases via its RING domain.
CNOT4 is phosphorylated in response to DNA damage at consensus sites recognized by ATM and ATR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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