Thermo Fisher Scientific PCNA Polyclonal Antibody
Rabbit polyclonal antibody targeting human PCNA, essential for DNA replication and repair. Validated for WB, IHC, Flow, and IP applications. Unconjugated liquid form, 1 mg/mL concentration, stored at 4°C. Recommended for research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000–1:10,000 |
| Flow Cytometry (Flow) | 0.03 µg/1×10⁶ cells in 150 µL |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 75 and 125 of human proliferating cell nuclear antigen |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, predicted to react with Rat, Chicken, and Bovine
Target Information
PCNA (polymerase delta auxiliary protein) is essential for DNA replication and involved in DNA excision and mismatch repair pathways. It binds to CDK inhibitor p21, endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).
PCNA serves as a marker for proliferating cells and is useful for assessing tumor proliferation status. It is localized in the nucleus, acts as a cofactor of DNA polymerase delta, and forms a homotrimer to enhance leading strand synthesis. Upon DNA damage, PCNA is ubiquitinated and participates in the RAD6-dependent DNA repair pathway.
Two transcript variants encoding the same protein have been identified, and pseudogenes exist on chromosome 4 and the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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