Thermo Fisher Scientific KAP-1 Polyclonal Antibody
KAP-1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC, IP 등 다양한 응용에 적합합니다. 고순도 항원 친화 크로마토그래피 정제, 1 mg/mL 농도, 4°C 보관. 인체 및 마우스 반응성.
- 카탈로그번호
- A300274A
- 판매단위
- pk
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Thermo Fisher Scientific KAP-1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:2,500 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1 and 50 of human Tripartite Motif-Containing 28 (TRIM28, UniProt ID: Q13263-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Notes:
- For ICC, formaldehyde fixation is recommended.
- Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
The KRAB (Kruppel-associated box) domain (~45 amino acids) is a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes have been identified in the human genome. These proteins repress transcription via interactions with KAP-1 (KRAB-associated protein 1), an 835 amino acid polypeptide containing a RING finger, B boxes, and a PHD finger.
KAP-1 forms complexes with KRAB-domain transcription factors to enhance repression efficiency. It directly interacts with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1), targeting KRAZ1 to centromeric heterochromatin foci containing HP1α, thus regulating transcriptional repression. Mutant KAP-1 unable to bind HP1 leads to random KRAZ1 distribution and strong transcriptional activation.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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