Thermo Fisher Scientific KEAP1 Polyclonal Antibody
Rabbit polyclonal antibody targeting human KEAP1 protein. Validated for WB, IHC (P), and ICC/IF applications. Recognizes cytoplasmic and nuclear KEAP1. Supplied as liquid form, suitable for research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 301–400 of human KEAP1 (NP_036421.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.268 mg/mL |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804684 |
Product Specific Information
- Positive Samples: MCF7, U-87MG, Jurkat
- Cellular Location: Cytoplasm, Nucleus
- Immunogen Sequence:
LVKIFEELTL HKPTQVMPCR APKVGRLIYT AGGYFRQSLS YLEAYNPSDG TWLRLADLQV PRSGLAGCVV GGLLYAVGGR NNSPDGNTDS SALDCYNPMT
Target Information
This gene encodes a protein containing KELCH-1-like and BTB/POZ domains. Kelch-like ECH-associated protein 1 (KEAP1) interacts with NF-E2-related factor 2 (Nrf2) in a redox-sensitive manner. Upon dissociation in the cytoplasm, Nrf2 translocates to the nucleus, inducing expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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