Thermo Fisher Scientific NIK Polyclonal Antibody
Thermo Fisher Scientific의 NIK Polyclonal Antibody는 인간 NIK 단백질을 인식하는 토끼 유래 폴리클로날 항체입니다. Western blot과 Immunocytochemistry에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로 NF-κB 신호전달 연구에 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A peptide corresponding to amino acids 931–947 of human NIK |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11156626 |
Product Specific Information
- A suggested positive control is 293 cell lysate.
- PA5-19871 can be used with blocking peptide PEP-0007.
Target Information
MAP3K14 (NIK) is structurally related to MAP3Ks. MAP3K14 phosphorylates and activates CHUK (IKKα) and IKBKB (IKKβ), which in turn phosphorylate IkBα, releasing NF-κB and leading to up-regulation of inflammatory response genes.
This kinase binds to TRAF2 and stimulates NF-κB activity. It shares sequence similarity with several other MAPKK kinases and participates in an NF-κB-inducing signaling cascade common to receptors of the TNF/NGF family and to the interleukin-1 type-I receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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