Thermo Fisher Scientific CD23 Monoclonal Antibody (B3B4), Super Bright 645, eBioscience

Thermo Fisher Scientific CD23 Monoclonal Antibody (B3B4), Super Bright 645, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
• Publications: View 7 publications

Product Specifications

Available Formats

• Unconjugated
• Biotin
• Brilliant UV 805
• eFluor 450
• eFluor 660
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The B3B4 monoclonal antibody reacts with mouse CD23, a 45 kDa type II transmembrane glycoprotein expressed on resting conventional B cells and modulated upon activation. B-1 cell lineage (CD5+ B cells) does not express CD23. Soluble CD23 forms are biologically active and function as low-affinity IgE receptors, playing a role in IgE response regulation and B-cell activation. CD21 is known to bind CD23.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes, ≤0.5 µg/test (100 µL final volume). Cell number may vary from 10⁵ to 10⁸ cells/test. Antibody titration recommended for optimal performance.

Fluorochrome Information:
Super Bright 645 is a tandem dye excited by the violet laser (405 nm) and emits at 645 nm. Use a 660/20 bandpass filter. Ensure instrument compatibility.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to reduce non-specific polymer interactions.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal brightness loss.

Excitation: 405 nm
Emission: 645 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD23 is a 45 kDa glycoprotein found on a subset of peripheral blood and tonsil B cells and highly expressed on EBV-transformed B lymphoblasts. It acts as the low-affinity IgE receptor on B cells and is involved in B cell growth, differentiation, and IgE production. Soluble CD23 is a potent mitogenic factor. CD23 dysfunction is associated with chronic conjunctivitis and chronic lymphocytic leukemia.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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