Thermo Fisher Scientific CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Yellow 610, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Yellow 610, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.1 µg)/test
Publications: -

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Product Specifications

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Additional Formats Available

• Alexa Fluor 532, 700
• APC, APC-eFluor 780
• Brilliant UV/Violet/Yellow series
• eFluor, FITC, PE, PerCP-eFluor, Super Bright series
• NovaFluor Blue/Red/Yellow variants
• Request custom conjugation available

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Product Specific Information

The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by thymocytes, mature T helper cells, and monocytes. CD4 serves as a receptor for HIV, and SK3 blocks HIV binding and mixed lymphocyte reactions. SK3 and RPA-T4 antibodies recognize distinct epitopes.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells. Use at 5 µL (0.1 µg) per test (100 µL final volume).

Note:
NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for compatibility.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining with NovaFluor conjugates. Add 5 µL per sample to antibody cocktails prior to cell mixing.

NovaFluor conjugates utilize Phiton technology based on nucleic acid dye structures, engineered for optimized spectral properties and minimal spillover.

Excitation: 552 nm
Emission: 612 nm
Laser: 561 nm (Yellow)

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Target Information

CD4 antigen is involved in recognition of MHC class II molecules and serves as a co-receptor for HIV. Expressed mainly on T helper cells, and also on monocytes, macrophages, and dendritic cells. Found in thymus, lymph nodes, tonsils, spleen, and certain non-lymphoid tissues. CD4 aids T-cell activation via association with TCR and Lck kinase. It may mediate neuronal damage in infectious and immune CNS diseases. Multiple alternatively spliced transcripts exist (RefSeq, July 2017).

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For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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