Thermo Fisher Scientific CD20 Monoclonal Antibody (L26), eFluor 615, eBioscience
CD20 단백질을 인식하는 L26 클론의 마우스 단일클론 항체로, eFluor 615 형광 표지. FFPE 조직 IHC에 적합하며, Rituximab 등 치료용 항체에 의해 차단되지 않음. 595/614 nm 형광 특성, 4°C 암암 저장. 연구용 전용 제품.
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Thermo Fisher Scientific CD20 Monoclonal Antibody (L26), eFluor 615, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | - |
| Immunohistochemistry (IHC) | - | 4 publications |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Cynomolgus monkey, Human, Mouse, Rhesus monkey |
| Published Species | Human, Non-human primate |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 615 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | L26 |
| Conjugate | eFluor™ 615 |
| Excitation / Emission Max | 595 / 614 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10853517 |
Available Formats:
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 514
- Alexa Fluor Plus 647
- Alexa Fluor Plus 750
- eFluor 660
- Custom conjugation upon request
Product Specific Information
The L26 monoclonal antibody recognizes human CD20, a two-transmembrane spanning protein expressed on precursor and mature B lymphocytes. Expression is lost upon differentiation to plasma cells. CD20 levels are important in evaluating malignant B lymphocytes, including Non-Hodgkin’s lymphoma and various leukemias. CD20 plays a role in B cell differentiation and activation.
The L26 antibody binds to an epitope in the cytoplasmic domain, requiring tissue permeabilization for staining. Unlike antibodies recognizing the extracellular domain, L26 binding is not blocked by therapeutic anti-CD20 antibodies such as Rituximab.
Applications Reported: Immunohistochemical staining of FFPE tissue sections (IHC-P).
Applications Tested: IHC on FFPE human tonsil with low pH antigen retrieval. Titration recommended for optimal performance. Not validated for flow cytometry.
Filter Recommendation:
Use filters capturing 615 nm emission (e.g., Excitation 560/55, 585LP, Emission 645/75). Alexa Fluor™ 594 filter acceptable.
Excitation: 595 nm
Emission: 615 nm
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD20 is a non-glycosylated surface phosphoprotein (33–37 kDa) expressed on mature and most malignant B cells, a subset of T lymphocytes, and follicular dendritic cells. It regulates calcium conductance, cell cycle progression, and B-cell proliferation. CD20 associates with lipid rafts and interacts dynamically with the B-cell receptor (BCR) upon activation.
CD20 remains on the cell surface following antibody treatment, making it a useful target for therapeutic B-cell depletion. Dysfunctions are associated with Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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