Thermo Fisher Scientific VISTA Recombinant Rabbit Monoclonal Antibody (BLR035F)
VISTA 단백질을 표적으로 하는 재조합 토끼 단클론 항체로, 인간 시료에서 반응성이 검증됨. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합. 고순도 정제 및 액상 형태로 제공되며 연구용으로만 사용 가능.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 - 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 - 1:500 |
| Flow Cytometry (Flow) | 2 µL per 1 × 10⁶ cells |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR035F |
| Immunogen | Peptide representing a region between residue 250 and the C-terminus (residue 311) of human V-type immunoglobulin domain-containing suppressor of T-cell activation precursor |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 100 µg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 7.8–8.6, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Target Information
VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is a product of the VSIR gene.
Although VISTA bears sequence homology to PD-L1, it has a distinct expression pattern. In both mouse and human, VISTA is predominantly expressed on hematopoietic cells—most abundantly on myeloid cells and at lower levels on T cells, while generally absent on B cells.
VISTA expressed by antigen-presenting cells directly suppresses proliferation and cytokine production of CD4 and CD8 T cells, making it a promising target for cancer immunotherapy.
Reported ligands include CD28H, VSIG-3, and VSIG-8.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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