Thermo Fisher Scientific CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Blue 585, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Blue 585

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.1 µg)/test

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Product Specifications

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Additional Available Formats

• Alexa Fluor 532, 700
• APC, APC-eFluor 780
• Brilliant UV/Violet/Yellow series
• eFluor 450, FITC, PE, PE-Cyanine5.5, PE-Cyanine7
• PerCP-eFluor 710, Super Bright series
• NovaFluor Blue/Red/Violet/Yellow variants
• Custom conjugation available upon request

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Product Specific Information

Description:
The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by thymocytes, T helper cells, and monocytes. CD4 serves as a receptor for HIV, and SK3 blocks HIV binding and mixed lymphocyte reactions. SK3 and RPA-T4 recognize distinct epitopes.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported:
Suitable for flow cytometric analysis.

Applications Tested:
Pre-titrated and validated for flow cytometry using normal human peripheral blood cells. Recommended usage: 5 µL (0.1 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.

NovaFluor Compatibility:
NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

CellBlox Plus Blocking Buffer:
Updated formulation (Cat. No. C001T06F01) enhances staining performance. Add 5 µL per sample before combining with cells. For single-color controls, use 5 µL per 100 µL cell sample.

Technology:
NovaFluor conjugates utilize Phiton technology, featuring engineered nucleic acid dye structures optimized for spectral performance and minimized spillover.

Optical Properties:
Excitation: 494 nm
Emission: 585 nm
Laser: 488 nm (Blue)

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Target Information

CD4 antigen mediates recognition of MHC class II molecules and acts as a co-receptor for HIV. Expressed mainly on T-helper cells, as well as monocytes, macrophages, and dendritic cells. Tissue expression includes thymus, lymph nodes, tonsils, spleen, and certain non-lymphoid tissues. CD4 participates in T-cell activation via Lck kinase association and may contribute to neuronal damage in infectious or immune-mediated CNS diseases. Multiple splice variants are known.

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For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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