Thermo Fisher Scientific Phospho-ULK1 (Ser758) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

Antibody detects endogenous levels of ULK1 only when phosphorylated at Ser757 of mouse ULK1 or Ser758 of human ULK1.

Target Information

ULK1 is a serine/threonine protein kinase that plays a critical role during the initial stages of autophagy, a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls regulatory function and localization. Knockdown of ULK1 inhibits autophagic response and rapamycin-induced autophagy, consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13, essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.