Thermo Fisher Scientific Phospho-ULK1 (Ser638) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Product Specifications

Product Specific Information

This antibody detects endogenous levels of ULK1 only when phosphorylated at Ser638.

Target Information

ULK1 is a serine/threonine protein kinase that plays a critical role during the initial stages of autophagy, a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response and rapamycin-induced autophagy, consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13, which is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for proper localization of ULK1 to the pre-autophagosome and for ULK1 protein stability. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.