Thermo Fisher Scientific CD20 Monoclonal Antibody (ZY492)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 2–10 µg/mL

Flow Cytometry (Flow)

• Tested Dilution: 0.5–5 µg/10^6 cells

Product Specifications

Product Specific Information

• Positive control: Thymus
• Cellular location: Membrane

Target Information

CD20 is a non-glycosylated surface phosphoprotein with a molecular weight of 33–37 kDa depending on phosphorylation. It is expressed on mature and most malignant B cells, a subset of T lymphocytes, and follicular dendritic cells. CD20 expression coincides with surface IgM and regulates calcium conductance, cell cycle progression, and B-cell proliferation. It associates with lipid rafts, with the intensity of association influenced by extracellular triggering and BCR aggregation. After receptor ligation, CD20 and BCR colocalize briefly before BCR endocytosis, while CD20 remains on the cell surface. CD20 is a key therapeutic target for antibody-mediated B-cell depletion as it is stably expressed on B-cell malignancies and does not internalize or shed upon antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.