ELK Biotechnology Abl1 (phospho Thr735) rabbit pAb

제품명

Abl1 (phospho Thr735) rabbit pAb

대체 명칭

ABL1; ABL; JTK7; Tyrosine-protein kinase ABL1; Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Proto-oncogene c-Abl; p150

적용 분야 (Applications)

• WB (Western Blot)
• IHC (Immunohistochemistry)
• IF (Immunofluorescence)
• ELISA

권장 희석 배수 (Recommended Dilutions)

• Western Blot: 1/500 - 1/2000
• Immunohistochemistry: 1/100 - 1/300
• ELISA: 1/5000
• Other applications: Not yet tested

면역원 (Immunogen)

The antiserum was produced against a synthesized peptide derived from human ABL1 around the phosphorylation site of Thr735.
Amino acid range: 701–750

제품 사양 (Specifications)

세포 내 위치 (Cellular Localization)

Cytoplasm, cytoskeleton, nucleus, and mitochondrion.
Shuttles between the nucleus and cytoplasm depending on environmental signals.
Sequestered into the cytoplasm through interaction with 14-3-3 proteins.
Localizes to mitochondria in response to oxidative stress.
Isoform IB: nucleus membrane, lipid-anchor. The myristoylated c-ABL protein is reported to be nuclear.

배경 (Background)

This gene is a proto-oncogene encoding a protein tyrosine kinase involved in various cellular processes such as cell division, adhesion, differentiation, and stress response.
Its activity is negatively regulated by the SH3 domain; deletion of this domain can result in oncogenic transformation.
The protein exhibits DNA-binding activity regulated by CDC2-mediated phosphorylation, suggesting a role in the cell cycle.
ABL1 is often fused with partner genes in leukemias, most notably in the t(9;22) translocation forming the BCR-ABL fusion gene.
Alternative splicing results in two transcript variants with different first exons spliced to common downstream exons.