Thermo Fisher Scientific Nrf2 Polyclonal Antibody
다양한 종에서 반응하는 Nrf2 폴리클로날 항체로, Western blot, IHC, ICC/IF, IP, ChIP 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제된 고품질 항체이며, 연구용으로만 사용. 장기 보관 시 -20°C에서 안정적으로 유지.
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Thermo Fisher Scientific Nrf2 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | 26 publications |
| Immunohistochemistry (IHC) | – | 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | 4 publications |
| Immunoprecipitation (IP) | 1:100–1:500 | 1 publication |
| ChIP assay (ChIP) | 5 µg | 1 publication |
| Miscellaneous PubMed (Misc) | – | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Avian, Human, Mouse, Plant, Reptile, Rat, Zebrafish |
| Published Species | Human, Mouse, Pig, Rat, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 108–413 of Human NRF2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.31 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545358 |
Product Specific Information
- Recommended positive controls: C8D30, 3xFlag-human NFE2L2-transfected 293T, MDA-MB-231 nuclear extract, MDA-MB-231 nuclear extract (30 µM tBHQ, 4 hr), HepG2 (10 µM MG132 treatment for 15 hr)
- Predicted reactivity: Rhesus Monkey (99%), Bovine (84%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Nfr1 (also designated TCF11), Nrf2, and Nfr3 are bZIP transcription factors that heterodimerize with Maf proteins to bind MARE (Maf recognition elements) sequences. Nrf2 is widely expressed and translocates to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from the repressor protein Keap1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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