Thermo Fisher Scientific PLS3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:10,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 66 and 371 of Human T-Plastin if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PLS3,uniProtId:P13797-1,ncbiNodeId:9606,antigenRange:66-371,antigenLength:630,antigenImageFileName:PA5-27883_PLS3_P13797-1_Rabbit.svg,antigenImageFileNamePDP:PA5-27883_PLS3_P13797-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2545359

Product Specific Information

Recommended positive controls: 293T, A431, JurKat, Raji, NIH-3T3, JC, BCL-1.

Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (89%), Xenopus laevis (91%), Chicken (95%), Rhesus Monkey (100%), Bovine (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Phospholipid scramblases (PLS) translocate phospholipids bidirectionally between inner and outer leaflets of the plasma membrane and are implicated in apoptosis (1,2). Members of this family, PLS1 and PLS3 are both substrates of Protein kinase C (PKC) delta and are phosphorylated by PKC-delta during apoptosis. PKC-delta translocates to mitochondria during apoptosis and phosphorylates PLS3. Overexpression of PLS3 in the HEK293 cells enhanced apoptosis induced by UV-irradiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.