
Thermo Fisher Scientific PLS3 Polyclonal Antibody
Thermo Fisher Scientific의 PLS3 Polyclonal Antibody는 인간 및 마우스 반응성을 가진 Rabbit IgG 기반 항체입니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 장기 보관 시 -20°C에서 안정적으로 유지됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:10,000 | View 4 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 3 publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 66–371 of Human T-Plastin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.23 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545359 |
Product Specific Information
- Recommended positive controls: 293T, A431, JurKat, Raji, NIH-3T3, JC, BCL-1
- Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (89%), Xenopus laevis (91%), Chicken (95%), Rhesus Monkey (100%), Bovine (95%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Phospholipid scramblases (PLS) translocate phospholipids bidirectionally between inner and outer leaflets of the plasma membrane and are implicated in apoptosis. Members of this family, PLS1 and PLS3, are substrates of Protein kinase C (PKC) delta and are phosphorylated by PKC-delta during apoptosis. PKC-delta translocates to mitochondria during apoptosis and phosphorylates PLS3. Overexpression of PLS3 in HEK293 cells enhances apoptosis induced by UV-irradiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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