Thermo Fisher Scientific IgM Monoclonal Antibody (eB121-15F9), PE, eBioscience
마우스 IgM의 μ 중쇄를 인식하는 단클론 항체로, 다른 면역글로불린 클래스와 반응하지 않음. Flow cytometry 및 ICC/IF에 적합하며 PE 형광표지로 검출 가능. 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 0.125 µg/test | 20 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eB121-15F9 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466012 |
Available Formats:
- Unconjugated
- Biotin
- eFluor 450
- FITC
- PE-Cyanine7
- Super Bright 436
- Super Bright 600
- Custom conjugation available
Product Specific Information
The eB121-15F9 monoclonal antibody reacts with the μ heavy chain of mouse IgM and does not cross-react with other mouse immunoglobulin classes (IgD, IgG, IgA). IgM is expressed intracellularly during early B cell development and on the surface of mature B cells in bone marrow and peripheral tissues.
Fluorochrome-conjugated eB121-15F9 can be used as a detection secondary antibody for mouse IgM.
Applications Reported:
- Flow cytometric analysis to detect surface IgM on mouse B cells
- Secondary detection of primary mouse IgMs
Applications Tested:
- Tested for flow cytometry at ≤ 0.125 µg/test (100 µL final volume)
- Recommended to titrate for optimal performance
- Suitable for 10⁵–10⁸ cells/test
Spectral Properties:
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Mouse IgM is an immunoglobulin M antibody produced by B cells in response to antigens. It is the first antibody generated during immune responses and is the largest immunoglobulin in serum. Composed of five heavy and five light chains, IgM mediates complement activation and antigen agglutination.
It plays a key role in the innate immune response, neutralizing pathogens before full activation of adaptive immunity. Mouse IgM is widely used in ELISA, Western blotting, and immunohistochemistry for research and diagnostic applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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