Thermo Fisher Scientific CD42b Monoclonal Antibody (HIP1), NovaFluor Blue 610-70S, eBioscience

Thermo Fisher Scientific CD42b Monoclonal Antibody (HIP1), NovaFluor Blue 610-70S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.2 µg)/test

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Product Specifications

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Additional Formats

• Unconjugated
• APC
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 660-120S
• NovaFluor Yellow 610
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

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Product Specific Information

The HIP1 monoclonal antibody reacts with the human CD42b molecule, a 135 kDa transmembrane protein also known as platelet gpIb.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-titrated and validated for flow cytometric analysis of human platelets.
Use at 5 µL (0.5 µg) per test (final volume 100 µL; cell number 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability analysis.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
• Add 5 µL per sample to antibody cocktails/master mixes prior to combining with cells.
• For single-color controls, use 5 µL of CellBlox per 100 µL cell sample.

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures optimized for minimal spillover and spread.

Optical Properties:

• Excitation: 509 nm
• Emission: 614 nm
• Laser: 488 nm (Blue)

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Target Information

CD42b is a platelet activation marker involved in coagulation and aggregation. It interacts with extracellular matrix and adhesion molecules and is regulated by VEGF and PDGF. CD42b forms part of the GPIb-IX-V receptor complex, essential for platelet-rich thrombus formation. It binds to von Willebrand factor, thrombin, coagulation factors XI/XII, high molecular weight kininogen, TSP-1, integrin Mac-1, and P-selectin. Expression occurs on platelets and megakaryocytes. Mutations in CD42b are associated with Bernard-Soulier syndrome and platelet-type von Willebrand disease.

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For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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