
Thermo Fisher Scientific Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 800
DyLight 800 형광 표지된 염소 유래 항-쥐 IgG(H+L) 2차 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합합니다. 교차흡착되어 비특이적 반응이 최소화되며, 높은 민감도와 신호 증폭 효과를 제공합니다.
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- SA510024
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- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Rat IgG-heavy and light chain |
| Conjugate | DyLight™ 800 |
| Excitation / Emission Max | 789 / 794 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556604 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, human, mouse, rabbit, and sheep |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to bovine, chicken, human, mouse, rabbit, and sheep.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins. They are useful in the detection, sorting, or purification of their specified targets.
Secondary antibodies provide versatility across detection systems (e.g., HRP, AP, fluorescence) and enhanced sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are produced by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats Available
- Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 647, 680
- AP, APC, Cyanine3, Cyanine5, DSB-X Biotin
- DyLight 488, 550, 594, 650, 680, 755
- FITC, HRP, PE, Texas Red-X, TRITC
- Custom conjugation available upon request
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