Thermo Fisher Scientific Goat anti-Rat IgM Cross-Adsorbed Secondary Antibody, DyLight 800
DyLight 800으로 표지된 염소 유래 항-랫 IgM 2차 항체로, 인간 및 마우스 반응 최소화. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합하며 높은 특이성과 민감도를 제공. 항원 친화 크로마토그래피로 정제됨.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:2,000 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Rat IgM |
| Conjugate | DyLight™ 800 |
| Excitation / Emission Max | 789 / 794 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556596 |
| Target | IgM |
| Cross Adsorption | Against human and mouse |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to human and mouse.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins. They are useful in detection, sorting, or purification of their specified targets. Secondary antibodies enhance versatility by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and increase sensitivity via signal amplification, as multiple secondary antibodies can bind to a single primary antibody. Typically, these antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to achieve high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 650
- DyLight 680
- DyLight 755
- Custom conjugation available
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