Thermo Fisher Scientific Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 650
DyLight 650 형광으로 표지된 염소 유래 항-Rat IgG (H+L) 2차 항체로, Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. 다양한 종에 대한 교차 반응 최소화. 높은 특이성과 신호 증폭을 제공하며 연구용으로 적합.
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Thermo Fisher Scientific Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 650
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Rat IgG-heavy and light chain |
| Conjugate | DyLight™ 650 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556601 |
| Target | IgG |
| Cross Adsorption | Bovine, chicken, human, mouse, rabbit, sheep |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to bovine, chicken, human, mouse, rabbit, and sheep.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins. They are useful for detection, sorting, or purification of the specified target.
Secondary antibodies provide versatility by enabling use with multiple detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity through signal amplification.
Typically generated by immunizing the host animal with pooled immunoglobulins from the target species, they can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, conjugation) to yield highly specific reagents.
Available Conjugate Formats
- Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 647, 680
- AP, APC, Cyanine3, Cyanine5, DSB-X Biotin
- DyLight 488, 550, 594, 680, 755, 800
- FITC, HRP, PE, Texas Red-X, TRITC
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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