Thermo Fisher Scientific Donkey anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 550
Thermo Fisher의 Donkey anti-Rat IgG (H+L) DyLight 550 항체는 랫트 IgG 검출용 교차흡착 2차 항체로, 높은 특이성과 낮은 비특이적 결합을 제공합니다. WB, IHC, ICC, Flow 등 다양한 응용에 적합하며, 556/570 nm 형광 특성을 가집니다.
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Applications and Tested Dilution
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Rat IgG-heavy and light chain |
| Conjugate | DyLight™ 550 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556607 |
| Target | IgG |
| Cross Adsorption | Bovine, chicken, goat, human, mouse, rabbit, sheep |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimum reactivity to bovine, chicken, goat, human, mouse, rabbit, and sheep.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins. They are useful for detection, sorting, or purification of rat antibodies.
Secondary antibodies provide versatility across detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, or label conjugation) to achieve high specificity.
Additional Formats
- DyLight 488
- DyLight 594
- DyLight 650
- DyLight 680
- DyLight 755
- DyLight 800
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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