Thermo Fisher Scientific TIN2 Polyclonal Antibody

Thermo Fisher Scientific TIN2 Polyclonal Antibody

Applications

• Western Blot (WB)

Tested Dilution: 1:500–1:2,000
Publications: - [References not provided]

Product Specifications

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is >95% (by SDS-PAGE).

Target Information

TRF1 is a negative regulator of telomere length maintenance. Two proteins, tankyrase and Tin2, which bind to mammalian TRF1, have been identified.
Tankyrase is a protein with homology to ankyrins and to the catalytic domain of poly (ADP-ribose) polymerase (PARP). It localizes to telomeres by binding to TRF1 through its ankyrin repeats and exhibits PARP activity. ADP-ribosylation of TRF1 reduces its ability to bind telomeric DNA, suggesting telomere function in human cells is regulated by poly (ADP)-ribosylation.
TIN2 was identified as a TRF1-interacting partner from a yeast two-hybrid cDNA library. It localizes to telomeres and is essential for proper regulation of telomere length. TIN2 and TRF1 are constitutively expressed, working together to counterbalance telomere elongation by telomerase. However, endogenous TIN2 may be difficult to visualize by immunostaining in certain cell systems.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.