Thermo Fisher Scientific Phospho-Smad3 (Ser204) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human SMAD3(Accession P84022), corresponding to amino acid residues around phosphorylated Ser204. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SMAD3,uniProtId:P84022-1,ncbiNodeId:9606,antigenRange:204,antigenLength:425,antigenImageFileName:PA5-104941_SMAD3_P84022-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104941_SMAD3_P84022-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816414

Product Specific Information

Antibody detects endogenous levels of Smad3 only when phosphorylated at Serine 204.

Target Information

SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.