Thermo Fisher Scientific Phospho-Smad3 (Ser425) Polyclonal Antibody
Phospho-Smad3 (Ser425) 폴리클로날 항체로 인간, 생쥐, 랫트 시료에서 사용 가능. Western blot, IHC, ICC/IF에 적합. Ser425 인산화된 Smad3만 검출. 고순도 정제 및 안정적 액상 형태로 제공.
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Thermo Fisher Scientific Phospho-Smad3 (Ser425) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human SMAD3 (Accession P84022), corresponding to amino acid residues around phosphorylated Ser425 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816412 |
Product Specific Information
Antibody detects endogenous levels of Smad3 only when phosphorylated at Serine 425.
Target Information
SMAD3 belongs to the SMAD family of proteins, similar to the gene products of the Drosophila mothers against decapentaplegic (Mad) and the C. elegans gene Sma.
SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.
This protein functions as a transcriptional modulator activated by transforming growth factor-beta (TGF-β) and is thought to play a role in the regulation of carcinogenesis.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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