Thermo Fisher Scientific S1P1 Recombinant Rabbit Monoclonal Antibody (JM10-66)
S1P1 단백질을 표적으로 하는 재조합 토끼 단클론 항체로, 높은 특이성과 민감도를 제공. Western blot, IHC, ICC/IF, Flow Cytometry 등 다양한 응용 가능. HEK293 세포에서 발현된 IgG 형 항체로 일관된 로트 간 품질 유지. 연구용으로만 사용 가능.
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Thermo Fisher Scientific S1P1 Recombinant Rabbit Monoclonal Antibody (JM10-66)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000–1:5,000 | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | View 1 publication |
| Flow Cytometry (Flow) | 1:50–1:100 | View publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM10-66 |
| Immunogen | Synthetic peptide within Human EDG1 aa 2–51 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809864 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
S1P1 is a G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P), coupled to the G(i) subclass of heteromeric G proteins. S1P1 signaling activates RAC1, SRC, PTK2/FAK1, and MAP kinases. It plays a key role in cell migration, actin cytoskeleton reorganization, and lamellipodia formation in response to sphingosine kinase SPHK1 activation. S1P1 is essential for normal chemotaxis toward sphingosine 1-phosphate, embryonic heart development, and cardiac morphogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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