Thermo Fisher Scientific Opsin 4 Polyclonal Antibody
Thermo Fisher Scientific Opsin 4 Polyclonal Antibody는 rat melanopsin을 검출하는 rabbit IgG 기반 항체입니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 1 mg/mL 농도의 액상 형태로 -20°C에서 보관합니다.
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Thermo Fisher Scientific Opsin 4 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 5 publications |
| Immunohistochemistry (IHC) | – | View 17 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:1,000 | View publications |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 9 publications |
| Miscellaneous PubMed (Misc) | – | View 2 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Rat |
| Published Species | Bat, Human, Mouse, Rat, Rodent, Shrew |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues E(455) Q K S K T P K T K R H L P S L D R R M(474) of rat melanopsin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2156044 |
Product Specific Information
PA1-781 detects rat melanopsin.
It has been successfully used in Western blot, immunofluorescence, and immunocytochemistry procedures.
By Western blot, this antibody detects ~53 and ~85 kDa proteins representing overexpressed unglycosylated and glycosylated melanopsin from HEK 293 cells (does not work well on endogenously expressed protein).
By immunofluorescence and immunocytochemistry, PA1-781 detects melanopsin from rat retinal ganglion cells (RGCs).
The immunogen is a synthetic peptide corresponding to residues E(455)–M(474) of rat melanopsin.
Target Information
Melanopsin is an opsin-like protein found in the retinal ganglion cells of mammals.
It is part of a secondary optical system that parallels the classic rod and cone system and reports directly to the suprachiasmatic nucleus, regulating circadian rhythms.
Melanopsin knockout mice show decreased capacity to entrain to light and dark cycles.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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