Thermo Fisher Scientific Opsin 4 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 5 publications 5 publications

Immunohistochemistry (IHC)

-

View 17 publications 17 publications

Immunohistochemistry (Frozen) (IHC (F))

1:1,000

-

Immunocytochemistry (ICC/IF)

5 µg/mL

View 9 publications 9 publications

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Rat

Published species

Bat, Human, Mouse, Rat, Rodent, Shrew

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues E(455) Q K S K T P K T K R H L P S L D R R M(474) of rat melanopsin. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Opsin 4,uniProtId:Q8R456-1,ncbiNodeId:10114,antigenRange:456-474,antigenLength:474,antigenImageFileName:PA1-781_Opsin_4_Q8R456-1_Rabbit.svg,antigenImageFileNamePDP:PA1-781_Opsin_4_Q8R456-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2156044

Product Specific Information

PA1-781 detects rat melanopsin.

PA1-781 has been successfully used in Western blot, immunofluorescence, and immunocytochemistry procedures. By Western blot, this antibody detects ~53 and ~85 kDa proteins representing overexpressed unglycosylated and glycosylated melanopsin from HEK 293 cells (does not work well on endogenously expressed protein). By immunofluorescence and immunocytochemistry, PA1-781 detects melanopsin from rat retinal ganglion cells (RGCs).

The PA1-781 immunogen is a synthetic peptide corresponding to residues E(455) Q K S K T P K T K R H L P S L D R R M(474) of rat melanopsin.

Target Information

Melanopsin is an opsin-like protein found in the retinal ganglion cells of mammals. Melanopsin is believed to be part of a secondary optical system that parallels that of the classic rod and cone system. This second system reports directly to the suprachiasmatic nucleus and is responsible for regulation of circadian rhythms. Melanopsin is believed to be the primary photopigment responsible for the regulation of these circadian rhythms, and Melanopsin knockout mice have been generated which demonstrate decreased capacity to entrain to light and dark cycles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.