Thermo Fisher Scientific Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine5.5, eBioscience
Armenian hamster 유래 IgG 동형 대조 항체로, Flow Cytometry용 음성 대조로 사용됩니다. PE-Cyanine5.5 형광체 결합으로 564/692 nm 파장에서 검출 가능하며, 비특이적 결합 평가에 적합합니다. 4°C 암소 보관, 실험용 전용 제품입니다.
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Thermo Fisher Scientific Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine5.5
Applications
- Flow Cytometry (Flow): Assay-Dependent
- Control (Ctrl): Assay-Dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Armenian hamster / IgG |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBio299Arm |
| Conjugate | PE-Cyanine5.5 |
| Excitation/Emission Max | 564 / 692 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11218505 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780, Biotin
- Brilliant UV series (395–805)
- Brilliant Violet series (421–786)
- eFluor 450, 506, 615, 660
- FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright series (436–780)
- Functional Grade
- Custom conjugation available upon request
Product Specific Information
The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested by flow cytometric analysis; use at same concentration as experimental antibody
- Excitation: 488–561 nm
- Emission: 695 nm
- Laser Compatibility: Blue, Green, Yellow-Green lasers
Target Information
Isotype control antibodies serve as negative controls to distinguish non-specific background signal from specific antibody binding.
They help identify background staining caused by Fc receptor binding, non-specific protein interactions, or cell autofluorescence.
Isotype controls are primarily used in flow cytometry, but also applicable to ChIP, immunohistochemistry, and gel shift assays.
To ensure accurate comparison, the control antibody should match the species and isotype of the primary antibody and use the same fluorophore label when directly conjugated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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