Thermo Fisher Scientific Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine5.5, eBioscience

Thermo Fisher Scientific Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine5.5

Applications

• Flow Cytometry (Flow): Assay-Dependent
• Control (Ctrl): Assay-Dependent

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780, Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–786)
• eFluor 450, 506, 615, 660
• FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright series (436–780)
• Functional Grade
• Custom conjugation available upon request

Product Specific Information

The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested by flow cytometric analysis; use at same concentration as experimental antibody
• Excitation: 488–561 nm
• Emission: 695 nm
• Laser Compatibility: Blue, Green, Yellow-Green lasers

Target Information

Isotype control antibodies serve as negative controls to distinguish non-specific background signal from specific antibody binding.
They help identify background staining caused by Fc receptor binding, non-specific protein interactions, or cell autofluorescence.
Isotype controls are primarily used in flow cytometry, but also applicable to ChIP, immunohistochemistry, and gel shift assays.
To ensure accurate comparison, the control antibody should match the species and isotype of the primary antibody and use the same fluorophore label when directly conjugated.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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