Thermo Fisher Scientific Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Biotin
Western blot, ICC/IF, Flow cytometry, ELISA 등 다양한 응용에 적합한 고감도 Biotin 결합 Donkey anti-Mouse IgG (H+L) 항체. 다중 교차흡착으로 비특이적 반응 최소화. Affinity 정제 및 안정적 PBS/BSA buffer 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534695 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat and sheep IgG |
| Antibody Form | Whole Antibody |
Available Conjugate Formats
- Alexa Fluor 350
- Alexa Fluor 488
- Alexa Fluor 546
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 647
- Alexa Fluor 680
- Alexa Fluor 790
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- Alexa Fluor Plus 680
- Alexa Fluor Plus 800
- AP
- FITC
- HRP
- TRITC
- Custom conjugation available upon request
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of target proteins. Secondary antibodies provide versatility for various detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and may undergo additional purification and modification (e.g., immunoaffinity chromatography, fragmentation, label conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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