Thermo Fisher Scientific Donkey anti-Rabbit IgG (H+L) Secondary Antibody, AP
AP 효소가 결합된 Donkey anti-Rabbit IgG(H+L) 2차 항체로, Western blot, IHC, ELISA 등에 사용 가능. 높은 특이성과 민감도를 제공하며, 친화 크로마토그래피로 정제됨. 4°C 보관, 장기 보관 시 -20°C 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 30 mM triethanolamine, 1% BSA, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534700 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Each lot of antibody is tested for sensitivity using ELISA and for specificity by immunoelectrophoresis (IEP).
Target Information
Anti-Rabbit secondary antibodies are affinity-purified with well-characterized specificity for rabbit immunoglobulins. They are useful for detection, sorting, or purification of rabbit antibodies. Secondary antibodies enhance versatility by allowing use with various detection systems (e.g., HRP, AP, fluorescence) and improve sensitivity through signal amplification. These antibodies are typically generated by immunizing the host with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Conjugate Formats
- Alexa Fluor 488
- Alexa Fluor 594
- Biotin
- FITC
- HRP
- Qdot 525
- Qdot 565
- Qdot 625
- Qdot 655
- TRITC
- Custom conjugation available upon request
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