Thermo Fisher Scientific Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Biotin
당나귀에서 유래한 항-토끼 IgG(H+L) 2차 항체로, 비오틴으로 결합되어 다양한 검출 시스템과 호환됩니다. ELISA, ICC/IF, 유세포분석 등에 사용 가능하며 높은 민감도와 특이성을 제공합니다. 친화 크로마토그래피로 정제되어 신뢰성 있는 결과를 제공합니다.
- 카탈로그번호
- A16027
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534701 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 594
- AP
- FITC
- HRP
- Qdot 525
- Qdot 565
- Qdot 625
- Qdot 655
- TRITC
- Request custom conjugation
Product Specific Information
Each lot of antibody is tested for sensitivity using ELISA and for specificity using immunoelectrophoresis (IEP).
Target Information
Anti-Rabbit secondary antibodies are affinity-purified and have well-characterized specificity for rabbit immunoglobulins. They are useful for detection, sorting, or purification of rabbit targets. Secondary antibodies enable the use of multiple detection systems such as HRP, AP, or fluorescence, and provide signal amplification since multiple secondary antibodies can bind to a single primary antibody. These antibodies are typically generated by immunizing the host with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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