Thermo Fisher Scientific CD45 Monoclonal Antibody (MEM-28)
인간 CD45 항원을 인식하는 Mouse monoclonal antibody (Clone MEM-28). 다양한 형식(WB, IHC, ICC, Flow Cytometry 등)에 사용 가능. 비결합형, Protein A 정제, 1 mg/mL 농도. PBS(pH 7.4) 내 15mM sodium azide 포함, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | - |
| Flow Cytometry (Flow) | 1 µg/mL | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEM-28 |
| Immunogen | Human thymocytes and T lymphocytes |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1072397 |
Available Conjugated Formats
- Alexa Fluor 488 (MA5-18141)
- Alexa Fluor 647 (MA5-18142)
- APC (MA1-19459)
- Biotin (MA1-19497)
- FITC (MA1-19569)
- PE (MA1-19635)
- PerCP (MA1-10230)
- Request custom conjugation
Product Specific Information
This antibody reacts with an extracellular epitope on all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180–220 kDa single chain type I transmembrane protein expressed at high levels on all hematopoietic cells except erythrocytes and platelets. This antibody does not cross-react with equine species.
- Western Blot: Non-reducing conditions; band around 200–250 kDa; 6% separating gel
- Immunohistochemistry (Paraffin): No pre-treatment of tissue sections required
- Immunocytochemistry: Paraformaldehyde fixation applicable
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells, comprising about 10% of lymphocyte surface proteins. It is absent on non-hematopoietic cells and tissues. CD45 plays a crucial role in lymphocyte development and antigen signaling by regulating Src-family kinases and JAK kinases. Multiple isoforms arise from alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic regions. CD45 is essential for T- and B-cell receptor signaling, cytokine receptor regulation, and integrin-mediated signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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