Thermo Fisher Scientific CD25 Monoclonal Antibody (MEM-181)
인간 CD25(IL-2Rα)에 특이적인 Mouse IgG1 단클론 항체로, 활성화된 B/T 세포 및 조절 T세포 표면의 CD25 발현 분석에 적합. Flow Cytometry에 검증됨. Protein A로 정제된 액상 포맷이며 4℃ 보관. 다양한 형광 표지 형식으로 제공 가능.
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Applications
- Flow Cytometry (Flow): Tested at 2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEM-181 |
| Immunogen | PHA-activated peripheral blood leucocytes |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1071770 |
Additional Formats
- Alexa Fluor 700 (Cat. MA5-28555)
- APC (Cat. MA1-19457)
- Biotin (Cat. MA1-19493)
- FITC (Cat. MA1-19565)
- PE (Cat. MA1-19633)
- PerCP (Cat. A15802)
- Custom conjugation available upon request
Product Specific Information
This antibody reacts with an extracellular epitope of CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages, and CD4⁺ T regulatory cells. Expression is lost on resting B and T lymphocytes.
Target Information
CD25 (IL2RA) encodes the alpha chain of the interleukin-2 receptor complex, which plays a key role in T and B cell proliferation. The IL2 receptor complex consists of IL2RA, IL2RB, and IL2RG subunits forming a high-affinity receptor.
IL2 acts as a growth and differentiation factor for lymphocytes, stimulates NK cell activity, and regulates immune responses. Mutations in IL2RA are associated with interleukin-2 receptor alpha deficiency, implicating its role in immune regulation and tolerance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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