Thermo Fisher Scientific Brevican Monoclonal Antibody (N294A/6), PerCP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

N294A/6

Immunogen

Fusion protein amino acids 219-655 of rat Brevican if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Brevican,uniProtId:P55068-1,ncbiNodeId:10114,antigenRange:219-655,antigenLength:883,antigenImageFileName:MA5-45598_Brevican_P55068-1_House_mouse.svg,antigenImageFileNamePDP:MA5-45598_Brevican_P55068-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

PerCP PerCP PerCP

View additional formats

• Unconjugated
• APC
• FITC
• PE
• Request custom conjugation

Excitation/Emission Max

482/675 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932052

Product Specific Information

1 µg/mL of MA5-45598 was sufficient for detection of Brevican in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 140kDa (and smaller due to proteolytic cleavage).

This antibody was formerly sold as clone S294A-6.

Target Information

Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains. It is also is secreted as a 145 kD core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55 kD N-terminal fragment is formed that contains the unique C terminal epitope EAMESE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.