Thermo Fisher Scientific CYLD Monoclonal Antibody (2C3)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

10 µg/mL

-

ELISA (ELISA)

0.1 ng/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

2C3

Immunogen

CYLD (AAH12342, 854 a.a. approximately 953 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CYLD,uniProtId:Q9NQC7-1,ncbiNodeId:9606,antigenRange:854-953,antigenLength:956,antigenImageFileName:H00001540-M01_CYLD_Q9NQC7-1_House_mouse.svg,antigenImageFileNamePDP:H00001540-M01_CYLD_Q9NQC7-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: QNMELFAVLC IETSHYVAFV KYGKDDSAWL FFDSMADRDG GQNGFNIPQV TPCPEVGEYL KMSLEDLHSL DSRRIQGCAR RLLCDAYMCM YQSPTMSLYK

Target Information

CYLD is a 956 aa, cytoplasmic, deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family of proteins with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, a proline rich region, a SH3 binding domain and a sequence homology to catalytic domain of UCH. CYLD is identified as a tumor suppressor protein affecting the JNK signaling pathway. CYLD is a negative regulator of TRAF2 and NF-kappa-B signaling pathway and is also known to have receptor-dependent role in regulating the I-kappa-B kinase pathway. It also has a deubiquitinating activity that is directed towards non-Lys-48-linked polyubiquitin chains and TRAP1 is a novel substrate for deubiquitination. Mutated CYLD is known to be associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.