Thermo Fisher Scientific CXCL2 Polyclonal Antibody, Biotin, PeproTech
Rat GRO-beta/MIP-2(CXCL2)에 특이적인 Biotin 결합 폴리클로날 항체. Western blot과 ELISA에 적합하며, 항원 친화 크로마토그래피로 정제됨. 동결건조 형태로 -20°C 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.25–1.0 µg/mL | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Published Species | Rat |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Rat GRO-beta/MIP-2 (CXCL2) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930747 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
VVVASELRCQ CLTTLPRVDF KNIQSLTVTP PGPHCAQTEV IATLKDGHEV CLNPEAPLVQ RIVQKILNKG KAN
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat GRO-beta/MIP-2 (CXCL2). The anti-Rat GRO-beta/MIP-2 (CXCL2)-specific antibody was purified by affinity chromatography and subsequently biotinylated.
Sandwich ELISA:
To detect Rat GRO-beta/MIP-2 by sandwich ELISA (100 µL/well antibody solution), use 0.25–1.0 µg/mL of this antibody. In conjunction with PeproTech Polyclonal Anti-Rat GRO-beta/MIP-2 (500-P75) as a capture antibody, it enables detection of at least 0.2–0.4 ng/well of Recombinant Rat GRO-beta/MIP-2.
Western Blot:
To detect Rat GRO-beta/MIP-2 by Western blot, use 0.1–0.2 µg/mL of this antibody. When used with compatible secondary reagents, the detection limit for Recombinant Rat GRO-beta/MIP-2 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Packaging:
500-P75BT-1MG is supplied as 2 × 500 µg.
Target Information
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. It is a hematoregulatory chemokine that suppresses hematopoietic progenitor cell proliferation in vitro. GRO-beta(5–73) exhibits highly enhanced hematopoietic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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