Thermo Fisher Scientific CXCL2 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
VVVASELRCQ CLTTLPRVDF KNIQSLTVTP PGPHCAQTEV IATLKDGHEV CLNPEAPLVQ RIVQKILNKG KAN

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat GRO-beta/MIP-2 (CXCL2). The anti-Rat GRO-beta/MIP-2 (CXCL2)-specific antibody was purified by affinity chromatography and subsequently biotinylated.

Sandwich ELISA:
To detect Rat GRO-beta/MIP-2 by sandwich ELISA (100 µL/well antibody solution), use 0.25–1.0 µg/mL of this antibody. In conjunction with PeproTech Polyclonal Anti-Rat GRO-beta/MIP-2 (500-P75) as a capture antibody, it enables detection of at least 0.2–0.4 ng/well of Recombinant Rat GRO-beta/MIP-2.

Western Blot:
To detect Rat GRO-beta/MIP-2 by Western blot, use 0.1–0.2 µg/mL of this antibody. When used with compatible secondary reagents, the detection limit for Recombinant Rat GRO-beta/MIP-2 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Packaging:
500-P75BT-1MG is supplied as 2 × 500 µg.

Target Information

Produced by activated monocytes and neutrophils and expressed at sites of inflammation. It is a hematoregulatory chemokine that suppresses hematopoietic progenitor cell proliferation in vitro. GRO-beta(5–73) exhibits highly enhanced hematopoietic activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.