Thermo Fisher Scientific UNG Polyclonal Antibody
Thermo Fisher Scientific의 UNG Polyclonal Antibody는 인간 UNG 단백질을 인식하는 토끼 폴리클로날 항체로, Western blot 및 ICC/IF에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 다양한 종에 예측 반응성을 보입니다. 연구용으로만 사용됩니다.
- 카탈로그번호
- PA534823
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific UNG Polyclonal Antibody
Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:3,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 44–273 of UNG (Uniprot ID: P13051) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.71 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2552175 |
Product Specific Information
- Recommended positive controls: HeLa, HeLa transfect shUNG #1, HeLa transfect shUNG #2
- Predicted reactivity: Mouse (91%), Rat (91%), Xenopus laevis (81%), Chicken (87%), Chimpanzee (100%), Bovine (97%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase. These forms are generated by alternative splicing and the use of two differentially regulated promoters (PA and PB).
The cDNAs for UNG1 and UNG2 are of similar size (2061 and 2058 bp, respectively), and thus the corresponding mRNAs are not resolved as two species in gel electrophoresis.
Nuclear UNG2 differs from mitochondrial UNG1 in 44 amino acids of the N-terminal sequence that is not necessary for catalytic activity.
A major role of the UNG gene products is to repair mutagenic U:G mispairs caused by cytosine deamination. For example, UNG2 removes misincorporated dUMP residues.
The level and expression pattern of UNG1 and UNG2 differ between cell and tissue types. Expression of UNG is cell cycle regulated and generally higher in proliferating tissues and cells.
UNG1 is a 304 amino acid protein, and UNG2 is a 313 amino acid protein.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.