Thermo Fisher Scientific Phospho-Cdc25A (Ser76) Polyclonal Antibody
Cdc25A 단백질의 Ser76 인산화 부위를 인식하는 rabbit polyclonal antibody. Western blot 및 IHC(Paraffin)에 적합하며, Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태로 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human Cdc25A around the phosphorylation site of Serine 76 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553674 |
Product Specific Information
This antibody detects endogenous protein at molecular weights of approximately 59 and 70 kDa.
Purity is greater than 95% by SDS-PAGE.
Target Information
CDC25A is a member of the CDC25 family of phosphatases, which includes CDC25A, CDC25B, and CDC25C. These phosphatases function at different phases of the cell cycle.
CDC25A is essential for progression through the G1 and S phases and activates cyclin-dependent kinase CDC2 by removing two phosphate groups.
In response to DNA damage, CDC25A is specifically degraded to prevent abnormal cells from dividing.
CDC25A is considered an oncogene, although its precise role in oncogenesis remains under investigation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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