Thermo Fisher Scientific CD8A Recombinant Rabbit Monoclonal Antibody (CD8, 4391R)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• For Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
• For formalin-fixed tissues: Heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes.

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin family involved in T cell signal transduction, differentiation, and activation.
CD8 is mainly expressed on T cells as a disulfide-linked heterodimer (CD8α/CD8β) and functions as a co-receptor with TCR for MHC class I molecules.
It also exists as CD8α homodimers on subsets of T cells, NK cells, monocytes, and dendritic cells.
Upon MHC-I/peptide complex ligation, CD8 recruits Lck kinase, leading to lymphokine production, motility, and CTL activation.
Activated CTLs are critical for eliminating pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is promoted by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.