Thermo Fisher Scientific Glypican 1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500- 1:2,000

-

Immunohistochemistry (IHC)

10 µg/mL

-

ELISA (ELISA)

1:10,000-1:50,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-Glypican-1 protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human glypican-1 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.1 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is specific for human glypican-1 protein. A BLAST analysis was used to suggest partial cross-reactivity with glypican from rat, mouse, Macaque, dog, cattle, and opossum sources based on 100 - 88% homology with the immunizing sequence. Cross-reactivity with glypican from other sources has not been determined.

Target Information

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.