Thermo Fisher Scientific Perlecan Monoclonal Antibody (A74)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (Frozen) (IHC (F))

1:100

-

ELISA (ELISA)

1:2,000

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

A74

Immunogen

Bovine corneal endothelial cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.07 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1086587

Product Specific Information

CSI 001-74-02 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin). CSI 001-74 cross-reacts with human perlecan. Other species have not been tested.

CSI 001-74-02 has been successfully used in ELISA, immunohistochemistry and Western blotting techniques. CSI 001-74-02 can be used in immunoprecipitation, ELISA and immunostaining of frozen PLP-fixed sections of bovine and human tissues. CSI 001-74-02 can be used in affinity chromatography, in conjunction with CSI 001-71-02 to separate recombinant domain I from full length perlecan.

Epitope of CSI 001-74-02 is located in domain V.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

Target Information

Perlecan is a major heparan-sulfate proteoglycan (HSPG) found within all basement membranes and cell surfaces. Because of its strategic location and ability to store and protect growth factors, perlecan has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a coreceptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.