Thermo Fisher Scientific Beta-catenin Monoclonal Antibody (OTI11H9), TrueMAB
Beta-catenin 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI11H9). Western blot 및 Flow cytometry에 최적화. Human 시료 반응성. Lyophilized 형태로 제공되며, 재구성 후 약 1 mg/mL 농도 유지. 세포 접착 및 신호전달 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI11H9 |
| Immunogen | Full length human recombinant protein of human CTNNB1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Beta-catenin is an adherens junction (AJ) protein involved in cell-cell adhesion and signaling regulation. It interacts with E-cadherin and alpha-catenin to anchor adhesion complexes to the actin cytoskeleton.
Beta-catenin also associates with the adenomatous polyposis coli (APC) protein and participates in Wnt/Wingless signaling pathways, influencing morphogenesis and cell proliferation.
Aberrant beta-catenin activity has been linked to various cancers, including colorectal, ovarian, and skin tumors. In the nucleus, it co-activates Lef/Tcf transcription factors to promote genes such as cyclin D and c-myc.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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