Thermo Fisher Scientific TIGIT Recombinant Mouse Monoclonal Antibody (MBSA43)

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

MBSA43

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor Plus 488
• Alexa Fluor Plus 555
• Alexa Fluor Plus 594
• Alexa Fluor Plus 647
• Request custom conjugation

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2942255

Product Specific Information

Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.

Description: The MBSA43 mouse monoclonal recombinant antibody reacts with human T cell immunoreceptor with Ig and ITIM domains (TIGIT), which is also known as VSTM3 or WUCAM. This 26 kDa protein is a member of the poliovirus receptor (PVR) family. The expression of TIGIT has been reported on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ T cells, and CD8+ T cells, but it is not expressed on B cells or naive CD4+ T cells. TIGIT may be upregulated on naive CD4+ T cells upon activation. TIGIT can interact with certain members of the PVR and PVR-like families, including CD155, and also mediates the interaction of NK and T cells with antigen presenting cells, fibroblasts, and endothelial cells that express PVR and PVR-like proteins.

MBSA43 is reported to crossreact with Rhesus macaque.

Applications Tested: This MBSA43 antibody has been tested by flow cytometric analysis of human PBMCs. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.