Thermo Fisher Scientific CD45RB Monoclonal Antibody (PD7/26), eFluor 660, eBioscience
CD45RB 단백질을 인식하는 마우스 단클론 항체로, eFluor 660 형광체가 결합되어 있습니다. 인간 시료에서 Flow Cytometry, IHC, ICC, Western blot 등에 사용 가능합니다. 4°C 암소 보관, 동결 금지. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 4–10 µg/mL | - |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | - |
| Flow Cytometry (Flow) | Assay-Dependent | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PD7/26 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11149345 |
Additional Formats
Product Specific Information
The monoclonal antibody PD7/26 recognizes CD45RB, an isoform of the Leukocyte Common Antigen (LCA, CD45). CD45RB is a protein tyrosine phosphatase receptor expressed on most hematopoietic cells and absent on non-hematopoietic cells. Expression is more restricted than CD45 and found on T-cell subsets, B cells, NK cells, and myeloid cells. Regulatory T cells (Tregs) express low levels of CD45RB, correlating with increased migration to infection sites. High CD45RB expression induces IL-2 and IFN-γ production instead of IL-4 in vitro.
Applications Reported: Flow cytometry, Western blotting, immunohistochemistry on FFPE tissue, and immunocytochemistry.
Applications Tested: Tested by IHC on FFPE tissue using IHC Antigen Retrieval Solution - Low pH (Product # 00-4955-58).
Fluorophore Information:
eFluor™ 660 replaces Alexa Fluor™ 647. It emits at 659 nm and is excited with the red laser (633 nm). Ensure instrument compatibility. Recommended use ≤10 µg/mL; titrate for optimal performance.
Excitation: 633–647 nm
Emission: 668 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD45RB is a receptor-type protein tyrosine phosphatase (CD45 glycoprotein). CD45 plays a key role in lymphocyte development and antigen signaling, regulating Src-family kinases, promoting cell survival via integrin-mediated pathways, and participating in DNA fragmentation during apoptosis.
CD45 isoforms differ in extracellular domains but share identical transmembrane and cytoplasmic domains. Expression varies by cell type and physiological state. CD45RB is expressed in microglia and inflammatory cells and is essential for immunoreceptor signaling and cell survival modulation. CD45 is expressed on all human hematopoietic cells except mature red blood cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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