Thermo Fisher Scientific CD366 (TIM3) Monoclonal Antibody (RMT3-23), PE, eBioscience
마우스 CD366(TIM3)을 인식하는 단클론 항체로, PE 형광체가 결합되어 흐름세포분석에 적합합니다. Th1/Tc1 세포 표면 단백질 검출에 사용되며, 0.5 µg/test로 최적화되었습니다. 565/576 nm 파장에서 여기/방출되며, 4°C 암소 보관이 권장됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.5 µg/test | View 36 publications |
| Functional Assay (Functional) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RMT3-23 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465973 |
Available Formats
- Unconjugated
- APC
- Brilliant Violet 421
- Brilliant Violet 605
- Brilliant Violet 650
- Brilliant Violet 711
- FITC
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 702
- Request custom conjugation
Product Specific Information
The RMT3-23 monoclonal antibody reacts with mouse CD366 (TIM3), a Th1-specific cell surface protein expressed by both BALB/c and C57BL/6 strains. CD366 is a type I transmembrane protein containing an immunoglobulin and mucin-like domain extracellularly and a tyrosine phosphorylation motif intracellularly. It is selectively expressed by differentiated CD4⁺ Th1 and CD8⁺ Tc1 cells, but absent on Th2 and Tc2 cells. Other hematopoietic cells such as naive T cells, B cells, macrophages, and dendritic cells do not express CD366 at the protein level.
CD366 expression increases at a late stage of Th1 differentiation, suggesting a role in effector function rather than differentiation. In experimental autoimmune encephalomyelitis (EAE) models, CD366 is expressed on most T cells in the CNS at disease onset, but minimally in peripheral tissues.
RMT3-23 has functional activity, blocking DC recognition of apoptotic cells and inducing autoantibody production.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse CD366-transfected cells at ≤0.5 µg/test (100 µL final volume). Optimal titration recommended.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green Lasers
Filtration: 0.2 µm post-manufacturing filtered
Target Information
TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2) is a 281 amino acid type I transmembrane glycoprotein expressed on terminally differentiated CD4⁺ Th1 and CD8⁺ Tc1 cells. It includes an IgV-like domain, mucin-like domain, and a conserved tyrosine phosphorylation motif. TIM3 regulates macrophage activation, autoimmune responses, and Th1-mediated immune tolerance. It has been implicated in experimental autoimmune encephalomyelitis (EAE) and other Th1-dependent autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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