Thermo Fisher Scientific CD262 (DR5) Monoclonal Antibody (MD5-1), PE, eBioscience
마우스 DR5(TRAIL-R2)를 인식하는 MD5-1 단클론 항체로, PE 형광표지되어 Flow Cytometry에 적합. TRAIL 매개 세포자멸사 연구에 활용 가능. 0.25 µg/test 권장, 4°C 암소 보관. 연구용 전용.
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Applications
- Flow Cytometry (Flow)
- Tested Dilution: 0.25 µg/test
- View 10 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MD5-1 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465999 |
Additional Formats
Product Specific Information
Description:
The MD5-1 monoclonal antibody reacts with mouse DR5 (also known as TRAIL-R2, Apo2, TRICK2, and KILLER). DR5 binds to TRAIL, activates NF-kappaB, and induces TRAIL-mediated apoptosis. DR5 mRNA is broadly expressed by normal tissues and human antigen is also expressed by some tumor cell lines.
Applications Reported:
- Flow cytometric analysis.
Applications Tested:
- Tested by flow cytometric analysis of mouse DR5-transfected cells.
- Recommended ≤ 0.25 µg per test (100 µL final volume).
- Cell number range: 10⁵–10⁸ cells/test.
- Antibody titration recommended for optimal results.
Fluorescence Characteristics:
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
Filtration:
- 0.2 µm post-manufacturing filtered.
Target Information
DR5 (Apo2, TRAIL-R2, TRICK2, KILLER):
A death domain-containing receptor for TRAIL, part of the TNF-receptor superfamily. DR5 mediates TRAIL-induced apoptosis and activates NF-kappaB signaling. Two transcript variants and one non-coding transcript are known. Highly expressed in lymphocytes and spleen.
Associated diseases include squamous cell carcinoma and diffuse infiltrative lymphocytosis syndrome.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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