Thermo Fisher Scientific Tyrosine Hydroxylase Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

1:1,000

View 9 publications 9 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:1,000

View 2 publications 2 publications

Immunohistochemistry - Free Floating (IHC (Free))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:1,000

-

Product Specifications

Species Reactivity

Human, Many, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Tyrosine Hydroxylase,uniProtId:P04177-1,ncbiNodeId:10114,antigenRange:1-498,antigenLength:498,antigenImageFileName:OPA1-04050_Tyrosine_Hydroxylase_P04177-1_Rabbit.svg,antigenImageFileNamePDP:OPA1-04050_Tyrosine_Hydroxylase_P04177-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 0.1mg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325653

Product Specific Information

In Western blot, this antibody detects a single ~60 kDa protein representing tyrosine hydroxylase from rat brain lysates of PC-12 cells stimulated by okadaic acid. Immunohistochemical staining of TH in human brain with OPA1-04050 results in intense labeling of the dopaminergic neurons in the substantia nigra.

Store at -20ºC short term, 80ºC long term.

Target Information

Tyrosine hydroxylase (TH) is an enzyme involved in the synthesis of catecholamine neurotransmitters dopamine, epinephrine, and norepinephrine. In all species, catecholamine synthesis is regulated by the interaction of TH with a cofactor, tetrahydrobiopterin (BH4). BH4 binds to the TH catalytic domain, resulting in enzymatic activity. Unlike TH in non-primate species, four human TH mRNA splice variants (hTH1-hTH4) have been isolated. These variants are identical in their catalytic domain, but differ in their N-terminal, regulatory domains. TH is also responsible for the conversion of L-tyrosine to L-dopa. TH plays a key role in the physiology of adrenergic neurons. The role of TH in the synthesis of catecholamine neurotransmitters suggests a correlation between the enzyme and a number of neuropathogenic diseases including: Parkinson`s disease, schizophrenia, Segawa syndrome, and dystonia, as well as a variety of cardiovascular diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.