Thermo Fisher Scientific CD314 (NKG2D) Monoclonal Antibody (1D11), eBioscience
인간 NKG2D(CD314)을 인식하는 1D11 단클론 항체로, NK 및 T세포 연구에 적합. Flow cytometry, IHC(F), IP, Neutralization 등 다양한 응용 가능. 0.5 mg/mL 농도의 액상 제품으로 PBS buffer에 보관. For Research Use Only.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC(F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 0.125 µg/test | 9 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Neutralization (Neu) | Assay-Dependent | 2 publications |
| Functional Assay | Assay-Dependent | 3 publications |
| Inhibition Assays (IA) | - | 1 publication |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1D11 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467660 |
Available Conjugated Formats
- APC (Cat. 17-5878-42)
- Biotin (Cat. 13-5878-82)
- Brilliant UV 615 (Cat. 366-5878-42)
- FITC (Cat. 11-5878-42)
- Functional Grade (Cat. 16-5878-82)
- PE (Cat. 12-5878-42)
- PE-Cyanine7 (Cat. 25-5878-42)
- PerCP-eFluor 710 (Cat. 46-5878-42)
- Super Bright 436 (Cat. 62-5878-42)
- Custom conjugation available upon request
Product Specific Information
Description:
The 1D11 monoclonal antibody reacts with human NKG2D, a 42 kDa lectin-like molecule expressed by NK cells, γδ T cells, CD8+ T cells, and some CD4+ T cells. NKG2D forms complexes with DAP10 and can costimulate multiple NK activation receptors. Its counter-receptors MICA/MICB are expressed on various epithelial tumors (lung, breast, kidney, ovary, prostate, colon carcinoma). 1D11 blocks soluble MICA binding to γδ TCR T cell clones and inhibits lysis by these cells, inducing redirected lysis of Fc receptor-bearing P815 cells.
Applications Reported:
Reported for use in flow cytometry, immunoprecipitation, and immunohistochemical staining of frozen tissue sections, as well as inhibition assays. Functional Grade Purified 1D11 (Product #16-5878) is recommended for functional assays. Fluorochrome-conjugated 1D11 is recommended for flow cytometry.
Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended ≤0.125 µg per test (100 µL final volume). Cell number may vary (10⁵–10⁸ cells/test). Antibody titration is advised for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Natural killer (NK) cells are lymphocytes that mediate lysis of tumor and virus-infected cells without prior activation. They regulate humoral and cell-mediated immunity and express several calcium-dependent (C-type) lectins involved in NK cell regulation. The NKG2 gene encodes a type II transmembrane protein with a C-type lectin domain, located within the NK gene complex containing multiple NK-specific lectin genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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